Honghui He

A group led by Dr.Prof. Honghui He, Tsinghua University, ShenZhen, China


Direction 1.1: Mueller matrix polarimetry and equipment, parameters analysis and calibration
Direction 1.2: Microstructures identification using Mueller matrix derived parameters
Direction 2.1: Clinical applications of Mueller matrix microscopy
Direction 2.2: In-vivo tissue imaging and quantitative detection using polarimetric parameters



Chao He†,*, Jintao Chang, Patrick S Salter, Yuanxing Shen, Ben Dai, Pengcheng Li, Yihan Jin, Samlan Chandran Thodika, Mengmeng Li, Aziz Tariq, Jingyu Wang, Jacopo Antonello, Yang Dong, Ji Qi, Jianyu Lin, Daniel S Elson, Min Zhang, Honghui He*, Hui Ma*, Martin J Booth*

Advanced Photonics, 2022, 2(4), 026001

We find that certain simpler vectorial metrics permit extraction of signatures that provide direct indicators of hidden optical properties of complex systems. We harness such characteristics to reveal the spin Hall effect of light, infer microscopic structure within laser-written photonic waveguides, and conduct rapid pathological diagnosis through analysis of healthy and cancerous tissue. This provides new insight for the broader usage of such asymmetric inferred vectorial information.

Chao He*, Honghui He*, Jintao Chang, Binguo Chen, Hui Ma, Martin J Booth*

Light: Science & Applications, 2021, 1(10), 1-20

We focus mainly on summarising methodologies and applications related to tissue polarimetry, with an emphasis on the adoption of the Stokes–Mueller formalism. Several recent breakthroughs, development trends, and potential multimodal uses in conjunction with other techniques are also presented. The primary goal of the review is to give the reader a general overview in the use of vectorial information that can be obtained by polarisation optics for applications in biomedical and clinical research.

Teng Liu, Min Lu, Binguo Chen, Qinsong Zhong, Jingyu Li, Honghui He*, Hua Mao*, Hui Ma*

Journal of Biophotonics, 2019, 12(12), e201900151

We apply the Mueller matrix microscopic imaging to the Crohn's disease (CD) and gastrointestinal luminal tuberculosis (ITB) tissue samples. The 2D Mueller matrix images of the CD and ITB tissue slices are measured using the Mueller matrix microscope developed in our previous study, then the Mueller matrix polar decomposition and Mueller matrix transformation parameters are calculated. We find the Mueller matrix derived parameters can reveal the structural features of tuberculosis areas and be used as quantitative indicators to distinguish between CD and ITB tissues, which may be useful for the clinical diagnosis.


★ Hui Ma, Honghui He, and Jessica Ramella-Roman. “Mueller matrix microscopy”, Polarized Light in Biomedical Imaging and Sensing: Clinical and Preclinical Applications 281-320, Springer International Publishing 2022.

★ Nan Zeng, Honghui He, Valery V. Tuchin, and Hui Ma. “Tissue optical clearing for Mueller matrix microscopy”, Handbook of Tissue Optical Clearing: New Prospects in Optical Imaging 1000, 31-66, CRC Press 2022.

★ 马辉, 何宏辉, 曾楠, 廖然.《全偏振测量与成像》, 科学出版社, 2022年11月第一版.





★ 马辉,黄彤宇,廖然,何宏辉,孟若愚,王晶,“一种透射式穆勒矩阵显微成像系统及方法”,中国,202011075354.4。

★ Hui Ma, Jintao Chang, Nan Zeng, Yonghong He, Ran Liao, Shuqing Sun, Honghui He, Zhenhua Chen, “Optical Polarisation Modulation and Detection Apparatus and Detection Method”, PCT/CN2015/070146, European Patent No. EP 3093636 B1. 30 Oct 2019. 欧洲

★ 马辉,何宏辉,孟若愚,“一种偏振成像方法及其装置、偏振成像系统”,中国,201910253142.1。(在审)

★ 程雪岷,余杰威,马辉,李懋林,何宏辉,“一种穆勒显微偏振像差校准方法及装置”,中国,201910185842.1。

★ Hui Ma, Jintao Chang, Honghui He, Nan Zeng, Yonghong He, Ran Liao, Shuqing Sun, Zhenhua Chen, “Light Polarization State Modulation and Detection Apparatuses and Detection Method”, PCT/CN2015/070146, U.S. Patent No. 9,976,906. 22 May 2018. 美国

★ 马辉,何超,常金涛,秦红伟,何宏辉,“一种偏振成像设备及方法”,中国,201510135076.X

★ 马辉,廖然,何宏辉,曾楠,何永红,“波长并行的流式细胞测量仪和测量方法”,中国,201310311426.4。

★ 马辉,廖然,何宏辉,曾楠,何永红,“频率并行的流式细胞测量仪和测量方法”,中国,201310312398.8。

★ 马辉,廖然,何宏辉,曾楠,何永红,“流式细胞测量仪和测量方法”,中国,201310312411.X。

★ 马辉,廖然,何宏辉,曾楠,何永红,“空间并行的流式细胞测量仪和测量方法”,中国,201310312385.0。

★ 郭继华,廖然,曾楠,马辉,何宏辉,“一种光扫描散射仪”,中国,201210524802.3。


★ Rui Hao, Nan Zeng, Zheng Zhang, Honghui He*, Chao He*, and Hui Ma. “Discrepancy of coordinate system selection in backscattering Mueller matrix polarimetry: exploring photon coordinate system transformation invariants”, Optics Express, 32(3): 3804-3816, 2024.

★ Rui Hao, Nan Zeng, Zheng Zhang, Honghui He*, Chao He, and Hui Ma. “Polarization feature fusion and calculation of birefringence dynamics in complex anisotropic media”, Optics Letters, 49(9): 2273-2276, 2024.

★ Wei Jiao†, Zheng Zhang†, Nan Zeng, Rui Hao, Honghui He*, Chao He*, and Hui Ma. “Complex spatial illumination scheme optimization of backscattering Mueller matrix polarimetry for tissue imaging and biosensing”, Biosensors, 14(4): 208, 2024.


★ Zheng Zhang, Rui Hao, Conghui Shao, Changjiang Mi, Honghui He*, Chao He*, E Du, Shaoxiong Liu, Jian Wu, and Hui Ma. “Analysis and optimization of aberration induced by oblique incidence for in-vivo tissue polarimetry”, Optics Letters, 48(23): 6136-6139, 2023.

★ Liangyu Deng†, Chunyan Chen†, Wenxi Yu†, Conghui Shao, Zan Shen, Yonggang Wang, Chao He*, Hongtao Li*, Zhiyan Liu*, Honghui He*, and Hui Ma. “Influence of hematoxylin and eosin staining on linear birefringence measurement of fibrous tissue structures in polarization microscopy”, Journal of Biomedical Optics, 28(10): 102909, 2023.

★ Zheng Zhang, Conghui Shao, Honghui He*, Chao He*, Shaoxiong Liu, and Hui Ma. “Analyzing the influence of oblique incidence on quantitative backscattering tissue polarimetry: a pilot ex vivo study”, Journal of Biomedical Optics, 28(10): 102905, 2023..

★ Yijie Shen*, Chao He*, Zipei Song, Binguo Chen, Honghui He, Yifei Ma, Julian AJ Fells, Steve J Elston, Stephen M Morris, Martin J Booth, and Andrew Forbes. “Topologically controlled multiskyrmions in photonic gradient-index lenses”, arXiv preprint arXiv:2304.06332, 2023.

★ Liangyu Deng†, Zhipeng Fan†, Binguo Chen, Haoyu Zhai, Honghui He*, Chao He*, Yanan Sun, Yi Wang, and Hui Ma. “A dual-modality imaging method based on polarimetry and second harmonic generation for characterization and evaluation of skin tissue structures”, International Journal of Molecular Sciences, 24: 4206, 2023.

★ 张钰新, 樊志鹏, 翟好宇, 何宏辉*, 王毅*, 何超, 马辉. 基于偏振成像的面部皮肤结构和特征非接触在体测量方法. 中国激光 50(3): 0307110, 2023.


★ Zhipeng Fan, Yong Zhou, Haoyu Zhai, Qi Wang, and Honghui He*. “A smartphone-based biosensor for non-invasive monitoring of total hemoglobin concentration in humans with high accuracy”, Biosensors, 12(10): 781, 2022.

★ Changjiang Mi†, Conghui Shao†, Honghui He*, Chao He*, and Hui Ma. “Evaluating tissue mechanical properties using quantitative Mueller matrix polarimetry and neural network”, Applied Sciences, 12(19): 9774, 2022.

★ Conghui Shao, Binguo Chen, Honghui He*, Chao He*, Yuanxing Shen, Haoyu Zhai, and Hui Ma. “Analyzing the influence of imaging resolution on polarization properties of scattering media obtained from Mueller matrix”, Frontiers in Chemistry, 10: 936255, 2022.

★ Yixuan Shi†, Yanan Sun†, Rongrong Huang, Yong Zhou, Haoyu Zhai, Zhipeng Fan, Zechao Ou, Pengsheng Huang, Honghui He*, Chao He*, Yi Wang*, and Hui Ma. “A quantitative technique to analyze and evaluate microstructures of skin hair follicles based on Mueller matrix polarimetry”, Frontiers in Physics, 10: 847656, 2022.

★ Si-Wei Zhang, Honghui He*, Chao He*, and Martin J. Booth*. “Editorial: Advances in nano-scale systems with optics (nano-chemical, nanomaterial, and nano-biomedicine)”, Frontiers in Chemistry, 10: 989153, 2022.

★ Yuanxing Shen†, Binguo Chen†, Chao He†, Honghui He*, Jun Guo, Jian Wu, Daniel Elson, and Hui Ma. “Polarization aberrations in high-numerical-aperture lens systems and their effects on vectorial-information sensing”, Remote Sensing, 14(8): 1932, 2022.

★ Chao He*†, Jintao Chang†, Patrick S. Salter†, Yuanxing Shen, Ben Dai, Pengcheng Li, Yihan Jin, Samlan Chandran Thodika, Mengmeng Li, Aziz Tariq, Jingyu Wang, Jacopo Antonello, Yang Dong, Ji Qi, Jianyu Lin, Daniel S. Elson, Min Zhang, Honghui He*, Hui Ma*, and Martin J. Booth*. “Revealing complex optical phenomena through vectorial metrics”, Advanced Photonics, 4(2): 026001, 2022.

★ Haoyu Zhai†, Yanan Sun†, Honghui He*, Binguo Chen, Chao He, Yi Wang*, and Hui Ma. “Distinguishing tissue structures via polarization staining images based on different combinations of Mueller matrix polar decomposition parameters”, Optics and Lasers in Engineering, 152: 106955, 2022.

★ Yaguang Ren, Ying Zhang, Honghui He, Liangjian Liu, Xiaojun Wu, Liang Song, and Chengbo Liu*. “Optical fiber-based handheld polarized photoacoustic computed tomography for detecting anisotropy of tissues”, Quantitative Imaging in Medicine and Surgery, 12(4): 2238, 2022.


★ Chao He*, Honghui He*, Jintao Chang, Binguo Chen, Hui Ma and Martin J. Booth*. “Polarisation optics for biomedical and clinical applications: a review”, Light: Science & Applications, 10: 194, 2021.

★ Pengcheng Li, Yang Dong, Jiachen Wan, Honghui He*, Tariq Aziz and Hui Ma*. “Polaromics: deriving polarization parameters from a Mueller matrix for quantitative characterization of biomedical specimen”, Journal of Physics D: Applied Physics, 55(3): 034002, 2021.

★ Binguo Chen†, Weipeng Li†, Honghui He*, Chao He*, Jun Guo, Yuanxing Shen, Shaoxiong Liu, Tao Sun, Jian Wu, and Hui Ma. “Analysis and calibration of linear birefringence orientation parameters derived from Mueller matrix for multi-layered tissues”, Optics and Lasers in Engineering, 146: 106690, 2021.

★ Yuanxing Shen†, Rongrong Huang†, Honghui He*, Shaoxiong Liu, Yang Dong, Jian Wu, and Hui Ma*. “Comparative study of the influence of imaging resolution on linear retardance parameters derived from the Mueller matrix”, Biomedical Optics Express, 12(1): 211-225, 2021.

★ Binguo Chen†, Yuxiang Lan†, Haoyu Zhai, Liangyu Deng, Honghui He*, Hua Mao*, and Hui Ma. “Comparative study of modified Mueller matrix transformation and polar decomposition parameters for transmission and backscattering tissue polarimetries”, Applied Sciences, 11: 10416, 2021.

★ Ruoyu Meng, Conghui Shao, Pengcheng Li, Yang Dong, Anli Hou, Chao Li, Liyan Lin, Honghui He, and Hui Ma*. “Transmission Mueller matrix imaging with spatial filtering”, Optics Letters, 46(16): 4009-4012, 2021.

★ Yuanhuan Zhu, Yang Dong, Yue Yao, Lu Si, Yudi Liu, Honghui He, and Hui Ma*. “Probing layered structures by multi-color backscattering polarimetry and machine learning”, Biomedical Optics Express, 12(7): 4324-4339, 2021.

★ Yang Dong, Jiachen Wan, Xingjian Wang, Jing-Hao Xue, Jibin Zou, Honghui He, Pengcheng Li, Anli Hou* and Hui Ma*. “A polarization-imaging-based machine learning framework for quantitative pathological diagnosis of cervical precancerous lesions”, IEEE Transactions on Medical Imaging, 40(12): 3728-3738, 2021.

★ Yang Dong, Jiachen Wan, Lu Si, Yixin Meng, Yanmin Dong, Shaoxiong Liu, Honghui He, and Hui Ma*. “Deriving polarimetry feature parameters to characterize microstructural features in histological sections of breast tissues”, IEEE Transactions on Biomedical Engineering, 68(3): 881-892, 2021.

★ Ruoyu Meng†, Zhenhua Chen†, Xingjian Wang, Yudi Liu, Honghui He, and Hui Ma*. “Comparison of different calibration methods for Mueller matrix microscopy of cells”, Applied Optics, 60(5): 1380-1386, 2021.


★ Yang Dong†, Shaoxiong Liu†*, Yuanxing Shen, Honghui He*, and Hui Ma*. “Probing variations of fibrous structures during the development of breast ductal carcinoma tissues via Mueller matrix imaging”, Biomedical Optics Express, 11(9): 4960-4975, 2020.

★ Yuanxing Shen, Wei Sheng, Honghui He*, Weipeng Li, and Hui Ma. “Assessing distribution features of fibrous structures using Mueller matrix derived parameters: a quantitative method for breast carcinoma tissues detection and staging”, Proceedings of SPIE, 11239: 112390F, 2020. (Invited Paper)

★ Pengcheng Li, Aziz Tariq, Honghui He, and Hui Ma*. “Characteristic Mueller matrices for direct assessment of the breaking of symmetries”, Optics Letters, 45(3): 706-709, 2020.


Honghui He*, Ran Liao, Nan Zeng, Pengcheng Li, Zhenhua Chen, Xi Liu, and Hui Ma*. “Mueller matrix polarimetry - an emerging new tool for characterizing the microstructural feature of complex biological specimen”, Journal of Lightwave Technology, 37(11): 2534-2548, 2019.

★ Teng Liu†, Min Lu†, Binguo Chen, Qinsong Zhong, Jingyu Li, Honghui He*, Hua Mao*, and Hui Ma*. “Distinguishing structural features between Crohn's disease and gastrointestinal luminal tuberculosis using Mueller matrix derived parameters”, Journal of Biophotonics, 12(12): e201900151, 2019.

★ Chao He*, Jintao Chang, Qi Hu, Jingyu Wang, Jacopo Antonello, Honghui He, Shaoxiong Liu, Jianyu Lin, Ben Dai, Daniel S. Elson, Peng Xi, Hui Ma, and Martin J. Booth*. “Complex vectorial optics through gradient index lens cascades”, Nature Communications, 10: 4264, 2019.

★ Wei Sheng†, Weipeng Li†, Ji Qi, Teng Liu, Honghui He*, Yang Dong, Shaoxiong Liu, Jian Wu, Daniel S. Elson*, and Hui Ma*. “Quantitative analysis of 4 × 4 Mueller matrix transformation parameters for biomedical imaging”, Photonics, 6(1): 34, 2019.

★ Aziz Tariq, Honghui He, Pengcheng Li, and Hui Ma*. “Purity-depolarization relations and the components of purity of a Mueller matrix”, Optics Express, 27(16): 22645-22662, 2019.

★ Da Li†, Fei Chen†, Nan Zeng*, Zhigang Qiu, Honghui He, Yonghong He, and Hui Ma*. “Study on polarization scattering applied in aerosol recognition in the air”, Optics Express, 27(12): A581-A595, 2019.


★ Tao Sun†, Teng Liu†, Honghui He*, Jian Wu, and Hui Ma*. “Distinguishing anisotropy orientations originated from scattering and birefringence of turbid media using Mueller matrix derived parameters”, Optics Letters, 43(17): 4092-4095, 2018.

★ Teng Liu†, Tao Sun†, Honghui He*, Shaoxiong Liu, Yang Dong, Jian Wu, and Hui Ma*. “Comparative study of the imaging contrasts of Mueller matrix derived parameters between transmission and backscattering polarimetry”, Biomedical Optics Express, 9(9): 4413-4428, 2018.

★ Ji Qi†, Honghui He†, Jianyu Lin, Yang Dong, Dongsheng Chen, Hui Ma*, and Daniel S. Elson*. “Assessment of tissue polarimetric properties using Stokes polarimetric imaging with circularly polarized illumination”, Journal of Biophotonics, 11(4): e201700139, 2018.

★ Jialing Zhou, Honghui He, Zhenhua Chen, Ye Wang, and Hui Ma*. “Modulus design multiwavelength polarization microscope for transmission Mueller matrix imaging”, Journal of Biomedical Optics, 23(1): 016007, 2018.

★ Pengcheng Li, Donghong Lv, Honghui He, and Hui Ma*. “Separating azimuthal orientation dependence in polarization measurements of anisotropic media”, Optics Express, 26(4): 3791-3800, 2018.

★ Yifan Fu, Zhiwen Huang, Honghui He, Hui Ma, and Jian Wu*. “Flexible 3 × 3 Mueller matrix endoscope prototype for cancer detection”, IEEE Transactions on Instrumentation and Measurement, 67(7): 1700-1712, 2018.


★ Yang Dong†, Honghui He†, Wei Sheng, Jian Wu, and Hui Ma*. “A quantitative and non-contact technique to characterise microstructural variations of skin tissues during photo-damaging process based on Mueller matrix polarimetry”, Scientific Reports, 7: 14702, 2017.

Honghui He, Chao He, Jintao Chang, Donghong Lv, Jian Wu, Chaijie Duan, Qian Zhou, Nan Zeng, Yonghong He, and Hui Ma*. “Monitoring microstructural variations of fresh skeletal muscle tissues by Mueller matrix imaging”, Journal of Biophotonics, 10(5): 664-673, 2017.

★ Yang Dong†, Ji Qi†, Honghui He*, Chao He, Shaoxiong Liu, Jian Wu, Daniel S. Elson*, and Hui Ma*. “Quantitatively characterizing the microstructural features of breast ductal carcinoma tissues in different progression stages by Mueller matrix microscope”, Biomedical Optics Express, 8(8): 3643-3655, 2017.

★ Ji Qi, Honghui He, Hui Ma*, and Daniel S. Elson*. “Extended polar decomposition method of Mueller matrices for turbid media in reflection geometry”, Optics Letters, 42(20): 4048-4051, 2017.

★ Dongsheng Chen, Nan Zeng, Qiaolin Xie, Honghui He, Valery V. Tuchin, and Hui Ma*. “Mueller matrix polarimetry for characterizing microstructural variation of nude mouse skin during tissue optical clearing”, Biomedical Optics Express, 8(8): 3559-3570, 2017.



Medical Physics and Physical Therapy is an interdisciplinary subject that applies the principles and methods of physics to the prevention, diagnosis, treatment and health care of human diseases. The content of physics courses is closely integrated with medicine, which can give full play to the value of physics concepts in medical education.



Dr.Prof. Honghui He

Honghui He received his PhD in physics from Tsinghua University, Beijing, China, in 2011. He is currently an associate professor with Tsinghua Shenzhen International Graduate School, Tsinghua University, China. His research interests include Mueller matrix polarimetric techniques and their applications in biomedical diagnosis, especially cancerous tissues’ detection. He is a member of SPIE, Optica, and the Biomedical Photonics Committee of Chinese Optical Society.

Group member

Yuxin Zhang

Liangyu Deng

Zheng Zhang

Rui Hao

Jiahao Fan

Yuzhu Shi

Haohan Zheng

Kaisha Deng

Wei Jiao


Chao He

Teng Liu

Weipeng Li

Yuanxing Shen

Rongrong Huang

Bingo Chen

Haoyu Zhai

Conghui Shao

Tao Xuan

Changjiang Mi

Zhipeng Fan

Yixuan Shi

Group photo



Institute of Biopharmaceutical and Health Engineering
Shenzhen International Graduate School
Tsinghua University